Managing A Ship Management Company

Question: Describe about the Managing A Ship Management Company. Answer: 1 Office Staff 1a.Location of office India Of the three locations namely Glasgow, Singapore and India shortlisted for setting up new office for Informal Ship management Ltd it decided to opt for India as the suitable location. It is based on two factors namely the cost of real estate property of the location and potential for ship management industry. Of the three location the cost per square foot per year is 115 US$ in Glasgow, 130 US$ in Singapore and 85 US$ in India and it is the last option India that provides cost advantage. In addition India holds the 16th position with a coastline of 7517 km and 13 major ports with 95% trade volume in maritime transport. 1b. Official language of the company India is multi linguistic country with many languages but Hindi and English are the most spoken language in the corporate world and majority of people understands, it[1]. In regard to Informal Ship management Ltd it is decided that English will be the Official language of the company. The reason for choosing the language is the parent company is based out of UK and the Indian location Chennai is more comfortable in English than Hindi so it acts as a more effective language for communication. 1cList of job title and members associated For Bulk carrier Job title Members associated Management Master Chief engineer Operational management Chief officer 2nd engineer 2nd officer 3rd engineer 3rd officer 4th engineer Electro Technical officer Supports Staffs Bonus/Store man chief cook OS/Greaser 11 steward AB/Greaser 1 cook For crew Job title Members associated Management Master Chief engineer Operational management Chief officer 2nd engineer 1st officer Cargo engineer 2nd officer 3rd engineer 3rd officer 4th engineer Electro Technical officer Supports Staffs Bonus/Store man chief cook OS/Greaser 11 steward AB/Greaser 1 cook 1d) Bulk Carrier Name Position held Nationality Gross Monthly Salary Gross annual salary Additional Costs(Transportation + training and fringe benefits) Master Indian 5600+1120+168=6888 82656 50+40+10=100 Chief Engineer English 4875+975+146.25=5996.25 71955 50+30+10=90 Chief Officer 2nd engineer Greek 3900+780+117=4797 57564 50+30+10=90 2nd officer 3rd engineer Scottish 2500+500+75=3075 36900 50+30+10=90 3rd officer 4th engineer Australian 1800+360+54=2214 26568 50+20+10=80 Electro technical officer Indian 2400+480+74.40=2954.4 31132.8 50+30+10=90 Chief cook Scottish 1225+245+36.75=1506.75 18081 50+20+10=80 Store man Indian 1225+245+36.75=1506.75 1808.1 20+10=30 Greaser I cook Scottish 1085+217+32.55=1334.55 16014.6 20+10=30 Greaser II steward French 870+174+26.10=1070.1 12840 20+10=30 Oil Tankers Name Position held Nationality Gross Monthly Salary Gross annual salary Additional Costs(Transportation + training and fringe benefits) Master Indian 5600+1120+168=6888 82656 50+40+10=100 Chief Engineer English 4875+975+146.25=5996.25 71955 50+30+10=90 Chief Officer 2nd engineer Greek 3900+780+117=4797 57564 50+30+10=90 1st officer cargo engineer English 3900+780+117=4797 57564 50+40+10=100 2nd officer 3rd engineer Scottish 2500+500+75=3075 36900 50+30+10=90 3rd officer 4th engineer Australian 1800+360+54=2214 26568 50+20+10=80 Electro technical officer Indian 2400+480+74.40=2954.4 31132.8 50+30+10=90 Chief cook Scottish 1225+245+36.75=1506.75 18081 50+20+10=80 Store man and pump man Indian 1225+245+36.75=1506.75 1808.1 20+10=30 Greaser I cook Scottish 1085+217+32.55=1334.55 16014.6 20+10=30 Greaser II steward French 870+174+26.10=1070.1 12840 20+10=30 1e) 1f Cost of the office per years area per feet 10 person reception broad room additional space Total space Sq feet 1200 288 240 1728 Cost of the person per years: - 85* 1728 =$146880 Additional costs for running the offcie = $36155 Additional costs for 10 persons=$361550 For bulk Total cost per year for one 10 persons =$508430 Total costs For 5 bulk carrier per years=$2542150 Monthly costs for office rental for 5 bulk carrier=$211846 For Oil tanker For Crew costs cost for office rental = $508430 Total costs for 5 crew costs per year =$2542150 Monthly costs for office rental=$211846 For both Yearly costs for both crew and bulk are = $2542150*2 =$5084300 Monthly costs for both crew and bulk are= $423691 2 Crew Cost 2a) identification of crewmembers and bulk carriers members Members for bulk carrier Job title Title No of employees Management Master 5 Chief engineer 5 TOTAL 10 Operational management Chief officer 2nd engineer 5 2nd officer 3rd engineer 5 3rd officer 4th engineer 5 Electro Technical officer 5 220 Supports Staffs Bonus/Store 5 man chief cook 5 OS/Greaser 11 steward 5 AB/Greaser 1 cook 5 20 Total members 50 For crew Job title Title No of employees Management Master 5 Chief engineer 5 TOTAL 10 Operational management Chief officer 2nd engineer 5 1st officer Cargo engineer 5 2nd officer 3rd engineer 5 3rd officer 4th engineer 5 Electro Technical officer 5 35 Supports Staffs Bonus/Store man chief cook 5 OS/Greaser 11 steward 5 AB/Greaser 1 cook 5 15 Total members 50 2b) Bulk Carrier Name Position held Nationality Gross Monthly Salary Gross annual salary Additional Costs(Transportation + training and fringe benefits) Total Master Indian 5600+1120+168=6888 82656 50+40+10=100 82756 Chief Engineer English 4875+975+146.25=5996.25 71955 50+30+10=90 72045 Chief Officer 2nd engineer Greek 3900+780+117=4797 57564 50+30+10=90 57654 2nd officer 3rd engineer Scottish 2500+500+75=3075 36900 50+30+10=90 36990 3rd officer 4th engineer Australian 1800+360+54=2214 26568 50+20+10=80 26648 Electro technical officer Indian 2400+480+74.40=2954.4 31132.8 50+30+10=90 31222.8 Chief cook Scottish 1225+245+36.75=1506.75 18081 50+20+10=80 18161 Store man Indian 1225+245+36.75=1506.75 18081 20+10=30 18111 Greaser I cook Scottish 1085+217+32.55=1334.55 16014.6 20+10=30 16044.6 Greaser II steward French 870+174+26.10=1070.1 12840 20+10=30 12870 Total 372502.4 Total cost for five ships = 372502.4*5=1862512 Oil Tankers Name Position held Nationality Gross Monthly Salary Gross annual salary Additional Costs(Transportation + training and fringe benefits) Total Master Indian 5600+1120+168=6888 82656 50+40+10=100 82756 Chief Engineer English 4875+975+146.25=5996.25 71955 50+30+10=90 72045 Chief Officer 2nd engineer Greek 3900+780+117=4797 57564 50+30+10=90 57654 1st officer cargo engineer English 3900+780+117=4797 57564 50+40+10=100 57664 2nd officer 3rd engineer Scottish 2500+500+75=3075 36900 50+30+10=90 36990 3rd officer 4th engineer Australian 1800+360+54=2214 26568 50+20+10=80 26648 Electro technical officer Indian 2400+480+74.40=2954.4 31132.8 50+30+10=90 31222.8 Chief cook Scottish 1225+245+36.75=1506.75 18081 50+20+10=80 18161 Store man and pump man Indian 1225+245+36.75=1506.75 18081 20+10=30 18111 Greaser I cook Scottish 1085+217+32.55=1334.55 16014.6 20+10=30 16044.6 Greaser II steward French 870+174+26.10=1070.1 12840 20+10=30 12870 Total 430166.4 Total cost for five ships = 430166.4*5=2150832 2c. Salary difference in Tanker and bulk carrier tour of duty Tanker tour of duty is normally less than that for bulk carriers and salaries are higher. Informal Ship management Ltd will continue this difference as the salary of the crew members are fixed based on the role and responsibility and not on the tour of duty[2]. They are different for staff working in tanker and bulk carrier with the former undertaking more risk than the later in terms of operation, extra machinery and cargo tank cleaning. 3 Management Fees 3a) affect of person employed and diversity of the staffs In present, 10 members have been selected to perform the operations for one site. Thereby, while operations functions properly then it may be required more staffs to perform operations effectively[3]. Thereby it is stated that this will affects numbers of person employed. As per the diversity concern, it has been seen that office will set up in India; thereby if organization hire more person are from Indian then work culture and performance will be improved. Overall, it has been stated that diversity has not much impact on organization performance. 3b) determine the services for supply and management fees Ship management costs Chennai Technical 4900 Crewing 2700 Fleet supports 1000 Shore premises costs 1000 Miscellaneous expenses 1000 Total costs monthly 107000 Total costs yearly 1284000 3c) Subtracting the annual salary for both the bulk and oil tankers together with the rental from the annual management fees 1284000-(1862512+2150832+2542150) = (-5271494) 3d Companys present state and changes required to increase profit Informal Ship management Ltd is new company and it is in the initial stage in the business life cycle. Thus the management of the company will aim for increasing the profit for the business of the company. The strategies taken for achieving the same will be focussing on the business development aspect both for bulk cargo and for tanker[4]. It will be supported by the operation efficiency of the staff involved and it will done by developing the skill and knowledge of the worker by rigorous training in handling bulk cargo and tanker. The company will focus on the bulk cargo in the Indian market as 95% of export in terms of volume and 70% in terms of value happen by the maritime and it provides huge opportunity for Informal Ship management Ltd to increase the profit and growth the business in the country. 4. From the above three tasks it is evident that the shipping management company named Informal Shipping Management Ltd will be launched in the Indian city, Chennai. This is based on the justification that the cost of the real estate property compared to Glassgow and Singapore of more attractive. In addition India has 13 ports and dispatches 95% of its cargo through the Maritime transport. Moreover, the business language has been chosen as English keeping in mind the linguistic comfort ability of the Chennai corporate culture. The list of job title identified for the bulk carrier are top management, operational management, support staff with job title like master, chief engineer, chief officer, electro technical officer, store man, chief cook and greaser. The same set of crew members are selected from the tanker business as well, the main difference is that the taker business include the cargo engineer. The salary component includes the gross salary, 20% of the full salary for national tax, 3% of the full salary for employee pension together with additional costs that include the transportation costs, training costs and the fringe benefits. The organigram has been depicted exhibiting the potential organizational structure that would be followed by the business. The cost of office per year amounts to 146880US$, the total costs for the bulk carriers amount to $2542150 and for the tanker it is 2542150. The amount that the company would have to pay as the salary for the members for bulk carriers amounts $1862512 and for the oil tanker amount to $2150832. The tanker tour of duty is less than the bulk carrier with high salary. It has been observed that the Informal Shipping Management Ltd also tends to follow the same trend since the tanker crew faces higher risk in terms of operation, extra machinery and cargo tank cleaning. Again, it has been observed that since the company emphasizes upon conducting all the businesses in house without sub contracting any business, the company in order to enhance the efficiency of its operations can recruit more staff members so, that the work load can be reallocated and each individual members would have to work as per their expertise which would allow them to carry out the tasks which are under their own expertise. The diversity would not be affected much. Moreover, the annual fees that would be achieved by the company by offering services like technical service, crewing service, fleet support, shore premises costs and miscellaneous expenses would amount $1284000 and after subtracting the annual salary for both the bulk and oil tankers together with the rental, it is observed that the company would be suffering from loss. The company present state can be defined as an initial stage in the business life cycle of the company and it aims to increase the profit in the fut ure. The company will use the strategy of developing the business and enhancing the skills and knowledge level of the operational staff. The focus of the company will be in the bulk cargo business as India exports 95% of cargo in terms of volume and 70% in terms value through maritime transport. Bibliography Perry, H. (2009).Ship management and operation. New York: Simmons-Boardman Pub. Co. Spruyt, J. (2007).Ship management. London: Lloyd's of London Press. Favre, D., Spruyt, J. and Willingale, M. (2008).Ship management. London: LLP. Guest, A. (2007).Ship management. 2nd ed. London: Lloyd's of London. Woodyard, D. (2009).Ship management. 4th ed. Colchester, Essex [England]: Lloyd's of London. Frankel, E. (2007). Ship management systems developments.Maritime Policy Management, 9(2), pp.135-143. Perry, H. (2009).Ship management and operation. New York: Simmons-Boardman Pub. Co. Dickie, J. (2009).Reeds 21st century ship management. 2nd ed.

Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Example

Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties Essay Introduction 1.1 Casein Milk contains about 30-35g protein /L. Approximately 80 % of which are present in casein micelles, these are big spherical composites incorporating 92 % protein and 8 % low molecular mass constituents, chiefly inorganic salts, chiefly calcium phosphate. Caseins represent four cistron merchandises IÂ ±s1-casein, IÂ ±s2-casein, I?-casein and I?-casein. Figure 1.1.1 shows the different protein types and their concentrations in milk. ( Advanced nutrient Chemistry A ) ( Advanced Food Chemistry A ) The precise construction of the casein is micelle has been subjected to legion scientific studied. Several theoretical accounts have been proposed over the last figure of old ages in order to depict casein micelle behavior. Caseins have distinguishable countries of positively and negatively charged groups in their primary constructions ensuing in amphiphilic belongingss. The caseins are known as rheomorphic proteins as they have highly flexible molecular construction. The caseins are thought to hold IÂ ±-helical or I?- sheet constructions, once more this is merely from theoretical surveies no such constructions have been found in the caseins because they have yet to be successfully crystallised. The secondary construction is loose and lacks order due to the high figure of proline residues which cause the protein concatenation to flex in a peculiar manner. Casein micelles are really stable against heat denaturation. As there is no third there is considerable exposure of hydrophobic residues, these consequence in strong association reactions and makes them comparatively indissoluble in H2O. All the caseins are conjugated proteins, most with one or more phosphate groups which are esterified to serine residues. These phosphate groups are of import to the construction of the casein micelle as Ca binding of the caseins is relative to the phosphate content. Table 1.1.2 shows some of the physicochemical features of the casein micelles. ( University G ) We will write a custom essay sample on Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Role of casein hydrolysate manufacturing conditions on hydrolysate technofunctional properties specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Physicochemical Features of Casein Micelle Diameter 50-300nm Surface Area 8 ten 10-10 cm2 Volume 2 ten 10-15 cm3 Density 1.063 g cm-3 Molecular weight ( hydrated ) 1.3 ten 109 Da Fullness 4.4cm3 per g protein Hydration 2g H2O per g protein Water Content ( hydrated ) 63 % ( Handbook ) ( Table 1.1.2 ) 1.2 Sodium Caseinate Manufacture ( Fig 1.2.1 ) ( Handbook ) Figure 1.2.1 gives a basic representation of the fabricating procedure of Na caseinate. The procedure involves foremost the separation of milk and pick, followed by pasteurisation. The caseins are so precipitated either by the add-on of a coagulator such as rennet or by a decrease in pH to 4.6 ( its isoelectric point ) . The curdled protein is heated to organize a curd. The curd is so separated from the whey by filtration or centrifugation in combination with counter-current rinsing with H2O. The curd is so reacted with an base, eg Na hydrated oxide and so dried to organize a caseinate. ( Handbook ) 1.3 Bioactive Peptides The primary construction of proteins consists of certain aminic acid sequences that have the ability to exercise physiological benefits in human existences. The amino acid sequences remain inactive when they are present as portion of the uninterrupted primary construction of the parent protein. However, when the parent protein is acted upon by an appropriate enzyme, the peptide is released ( Dr Rotimi Aluko ) . Enzymatic hydrolysis of milk proteins has been shown to cut down antigenicity, and increase biological activity for illustration by the release of immunomodulating, opioid and antihypertensive peptides. To this terminal, turning involvement has been focused on physiologically active peptides derived from milk proteins. In add-on, the little peptides present in protein hydrolysates are absorbed more quickly from the bowel than free amino acids or integral proteins. ( Spellman et Al ) . A sum-up of bioactive peptides which are derived from milk proteins and their maps are displa yed in figure 1.3.1 ( Hannu K ) ( Fig 1.3.1 ) 1.4 Enzyme readying The enzyme readying used for casein hydrolysis in this survey was Prolyve 1000a„? a commercially available protease readying which is of bacterial beginning. This readying contains the enzyme Subtilisin Carlsberg which is from a household of serine endopeptidases isolated from Bacillus licheniformis. Endopeptidases are enzymes which cleave within the protein or polypeptide concatenation. Subtilisin Carlsberg has a wide specificity for the hydrolysis of peptide bonds, with a penchant for a big uncharged residue. It is an aromatic enzyme with a penchant for carboxyl side cleavage. Hydrolyzes peptide amides incorporating leucine and tyrosine residues. This enzyme readying has been used in the hydrolysis of whey proteins ( Spellman et Al, 200 ) nevertheless to day of the month at that place does non look to be any publications utilizing this enzyme activity in the hydrolysis of casein substrates. 1.5 Resentment The chief disadvantage of protein hydrolysis is bitterness. Integral nutrient proteins due to their molecular size are improbable to interact with the taste-bud receptors and as such would non lend significantly to season ( Enzymology ) . As hydrolysis interruptions down these proteins into much smaller peptides interaction with taste-bud receptors can happen. Besides in Intact casein micelles the most hydrophobic amino acids are oriented towards the inside of the molecule, during hydrolysis peptides incorporating hydrophobic amino acids are released. As hydrolysis continues, more hydrophobic amino acid residues become exposed for this ground hydrolysate resentment by and large increases with increasing hydrolysis ( Spellman et Al ) . The Q-rule devised by ( Ney, 1971 ) established a quantitative relationship between the amino acerb composing of a peptide and its resentment. Using the values calculated by ( Tanford,1962 ) , the Q-rule stated that peptides with an mean hydrophobicity ( Q ) value greater than 1400calmola?’1 and with molecular multitudes below 6000Da elicit a acrimonious taste. ( Lemieux,1992 ) . Figure 1.5.1 shows several protein types and their Q values, it besides shows the different casein categorizations and their single Q values. I?-casein has a Q value of greater than 1400 kcal mol-1 this may slightly predispose to bitterness if during the hydrolysis the proteins are broken down to peptides weighing less than 6000Da ( Figure 1.5.1 ) Bitter peptides typically contain 3-15 amino acids and are characterised by the presence of hydrophobic aminic acids such as leucine, isoleucine, proline, valine, phenylalanine, tyrosine and tryptophan. ( Enzymology ) . The distinguishable acrimonious spirit of protein hydrolysates has been a major restriction in their usage in nutrient and wellness merchandises, they would necessitate to be incorporated into nutrients at really low concentrations to forestall its presence bring forthing an unacceptable spirit. 1.6 Enzymatic Hydrolysis of protein and the factors which affect it Enzymatic hydrolysis of protein is the procedure by which proteins are broken down by peptidases. Several factors affect the rate hydrolysis these include, enzyme specificity, extent of protein denaturation, enzyme: substrate ratio, entire solids concentrations, viscousness, pH, ionic strength, temperature and absence or presence of repressive substances. The specificity of an enzyme is a cardinal factor, act uponing both the figure and location of the peptide linkages that are hydrolyzed. Endopeptidases cleave the peptide linkage between two next amino acid residues in the primary sequence of a protein, giving two peptides. Proteolysis can continue either consecutive, by let go ofing one peptide at a clip, or through the formation of intermediates that are farther hydrolyzed to smaller peptides. ( Panyam et al, 1996 ) . There is really small information available about the consequence of entire solids on the rate of hydrolysis or the resulting belongingss of the hydrolysate samples. Spellman et Al, 2004 carried out a survey on whey protein hydrolysates and how entire solids affected the rate of the hydrolysis and the physiochemical belongingss of the end point hydrolysates. They concluded that the resentment of the hydrolysate samples decreased with increasing entire solids concentrations. Viscosity is the step of opposition to flux. Solvent viscousness can act upon rates of enzyme catalyzed reactions by two principle mechanisms: ( 1 ) Since molecular diffusion coefficients vary reciprocally with the viscousness of the medium, an addition in solvent viscousness will take to a lessening in the association rate of an enzyme and substrate. This will attest itself in a viscosity-dependent lessening in kc/Km for reactions in which the procedure that is governed by kc/Km is diffusion-controlled. ( 2 ) Since dissolver viscousness dampens structural fluctuations of proteins through frictional effects, additions in solvent viscousness will take to lessenings in reaction rates for catalytic procedures that are dependent on enzyme structural fluctuations Dang, 1998 this is supported by surveies from Gavish, 1979 and Ng, 1991 The consequence of temperature on the rate of enzymatic hydrolysis relates to the enzyme and the optimal conditions to ease its reaction but besides to the substrate where cautiousness must be taken in order to avoid /facilitate heat denaturation. Whichever of the conditions is preferred should be controlled. pH besides affects the rate of enzyme action and may present a menace of denaturation the enzyme. pH besides has an consequence on protein solubility. The iso-electric point is the pH at which the protein has no net charge, at this pH proteins would lose solubility and precipitate out of solution. Enzymes work better on proteins in solution. The ionic strength of the may impact the rate of hydrolysis. A solution of low ionic strength ( 0.5M 1.0M ions of impersonal salts ) may increase the solubility of the protein by salting in . Whereas concentrations above 1.0M may cut down the solubility of the protein as it causes increased competition for H2O molecules, favoring protein- protein interactions and thereby salting out . 1.7 TNBS Assay The method used to quantify the grade of hydrolysis ( DH ) of the Na caseinate hydrolysates was the trinitrobenzenesulfonic acid ( TNBS ) assay that was described by Adler-Nissen,1979 ) . Degree of hydrolysis ( DH ) is defined as the per centum of the entire figure of peptide bonds in the protein which have been cleaved by hydrolysis. The TNBS Assay was used in this undertaking as it has been proven to be a extremely accurate method for quantifying the DH of hydrolysate samples ( Spellman, 2003 ) . The one drawback of this method is that is requires long incubation and chilling stairss. This method is a spectrophotometric check of the chromophore formed by the reaction of the TNBS with primary Zanzibar copals. Figure 1.8.1 is a representation of the basic mechanism of how the TNBS check plants. Simply put the TNBS reagent binds to the NH2 group of the peptides in the sample and in making so creates a xanthous coloring material. The more hydrolyzed the sample is the more peptides there are and hence the more NH2 groups to adhere to and the more xanthous the solution will go. These solutions are so read spectrophotometrically . This check consists of several stairss foremost the protein hydrolsate is dispersed in hot 1 % Na dodecyl sulphate ( SDS ) , this serves to guarantee an accurate consequence as it prevents clip-clop of the protein and enables the TNBS reagent to split all of the NH2 groups. This reaction favours somewhat alkalic conditions ( pH 8.2 ) which is facilitated by the add-on of Na phosphate buffer. TNBS reagent reacts easy with hydroxyl ions and could do the space to give a false somewhat increased reading. This addition is stimulated by visible radiation and for this ground many of the stairss are carried out in the dark, such as the incubation for 1hour at 50Â °C and the subsequent expiration of the reaction which is achieved by take downing the pH. Termination is accomplished by the add-on of HCL, cautiousness must be taken to guarantee that the pH does non drop at a lower place 3.5 as this would causes turbidness. The samples are allowed to chill at room temperature for 30mins, chillin g below room temperature may besides do turbidness. After standing for 30mins the samples and their optical density readings are stable and more accurate. 1.8 Reverse Phase -High Performance Liquid Chromatography ( RP-HPLC ) Reverse-phase ( RP ) HPLC is an indispensable tool in the separation of proteins and peptides. RP-HPLC is widely used in protein surveies because of its versatility, sensitive sensing ( can divide proteins of about indistinguishable construction ) and its ability to work together with techniques such as mass spectroscopy. High public presentation liquid chromatography severs to heighten detectability of the analyte and can be applied to the analysis of any compound with solubility in a liquid that can be used as the nomadic stage ( Rounds, 1988 ) . The major constituents of a high public presentation liquid chromatography system include a pump, injector, column, sensor and information system. Change by reversal stage -HPLC is where the mutual oppositions of the stationary stage and the nomadic stage are reversed in comparing to the normal stage of soaking up chromatography ( Macrae, 1988 ) . The stationary stage is a solid support that is non-polar. Reversed stage media are composed of a base matrix to which organic ligands for surfacing silicon oxide such as octyl ( C8 ) or octadecyl groups ( C18 ) are attached ( Sofer, 1997 ) . The nomadic stage is a polar liquid that flows over the stationary stage. The sample is dissolved in the initial nomadic stage ( eg. Trifluoroacetic acid ) prior to being filtered and applied by injection to the column. Polar nomadic stages are normally H2O assorted with methyl alcohol, acetronitrile or trifluoroacetic acid ( Rounds, 1988 ) . The interaction of the constituents bring separated and the stationary stage rely on hydrophobic interactions and this determines the grade of migration in the column and separation of the constituents in the sample. Polar compounds are the first to be eluted as they are hydrophilic and have hebdomad interactions with the stationary stage. The pumps map is to present the nomadic stage through the system at a controlled flow rate of 1ml/min. Gradient elution system is used which involves two independent programmable pumps that are mixed at high force per unit areas ( Rounds, 1988 ) . This allows different compounds to be eluted by increasing the strength of the organic dissolver in a additive manner. The usage of a valve injector places the sample for dividing into the following nomadic stage and it is carried in this liquid for debut into the column. The sample injection is normally automated. The HPLC column is normally constructed of chromium steel steel tubing with eradicators that allow it to be connected between the injector and sensor of the system ( Rounds, 1988 ) . The packing stuff for this column is in the signifier of a chromatographic bed and acts as both a stationary stage and a support. The sensor for the HPLC is the constituent that emits a response due to the eluting sample compound/ concentration alterations in the column eluent and later signals a extremum on the chromatogram. Peptides do non absorb light above 220nm and soaking up of 214nm may be used to follow the concentration of peptides in the column wastewater ( Sofer, 1997 ) . Optical density of peptide at this wavelength is performed as peptides do non hold a 3-dimensional construction and all the amino acids are exposed and easy interact with the chromatographic media. Proteins don non hold the same interaction with the media as merely a little measure of its molecules tend to interact. Proteins usually show an optical density at 280nm due to the content of aromatic amino acid replacements ( Sofer, 1997 ) . Ultra violet sensing allows following of protein concentration of the wastewater and a chromatogram visually shows the extremums of the peptides and aromatic amino acids nowadays in sample. Column and nomadic stage temperature and pH can impact the separation of proteins and peptides by RP-HPLC. Increasing the temperature reduces the keeping of peptides. The temperature affects the comparative keeping of selectivity, which affects declaration ( David Carr ) . 1.9 Centripetal Evaluation Centripetal rating involves both rules of experimental design and statistical analysis. Centripetal rating of nutrient can utilize the human senses of gustatory sensation, touch, sight and odor to measure different facets of nutrient such as spirit, texture, visual aspect and olfactory property. For this undertaking the accent is on spirit and gustatory sensation will be the sense of pick. Flavour can be referred to as the esthesis perceived from nutrient or liquid taken in the oral cavity ( Fisher et al, 1997 ) . The four basic gustatory sensations are sweet, rancid, salty and bitter. For this undertaking a centripetal panel was selected and trained. Campaigners for the panel were selected foremost on the footing of being able to separate between Sweet, rancid, salty and bitter. Successful campaigners were so trained to observe and quantify resentment utilizing caffeine criterions. Statistical analysis of the resentment tonss was carried out utilizing the statistical programme R ver sion 2.10.1AÂ © , One-way analysis of discrepancy ( ANOVA ) and independent-samples t-tests were performed on centripetal informations. A important consequence was defined as P lt ; 0.05, a extremely important consequence was defined as P lt ; 0.001. Centripetal rating is a important facet in every undertaking whose ultimate end is to market a food/ functional nutrient merchandise. Bitterness is a major restricting factor in the production and incorporation protein hydrolysate into nutrients and centripetal rating is the most effectual method of finding the resentment degree. 1.10 Aims of this survey The aims of this survey are: O To bring forth Na caseinate hydrolysates at different protein/ entire solids concentrations. O Physicochemical word picture of the hydrolysate samples. O Selection and preparation of a centripetal panel to find the degree of resentment of the hydrolysates. O To find if the resentment of Na caseinate hydrolysates is related to protein/ entire solids concentration at which the hydrolysates were generated. Chapter 2 Materials and Methods 2 Materials and Methods 2.1 Materials Sodium caseinate was obtained from Kerry Group, Ireland and its protein content was determined to be 88.01 % utilizing Kjeldhal analysis. Prolyve 1000 was obtained from Lyven Enzymes Industrielles, Caen, France Trifluoroacetic acid ( TFA ) , HPLC class acetonitrile, L-leucine, HPLC grade H2O, Citric acid, Na chloride, sucrose and caffeine were obtained from Sigma Chemical Co. ( Poole, Dorset, UK ) . PuradiscTM 25 AS disposable syringe filters ( 0.2 AÂ µm ) , Supor_ hydrophilic membrane filters ( 47 millimeter, 0.2 lumen ) and 2N NaOH ( Titripur, SWR, 1.09136,1000 ) were obtained from VWR chemicals, Ireland. 2.2 Protein finding utilizing the Kjeldahl process The Kjeldhal method for finding protein concentration is an accretion of several reactions, the first of which is digestion, where protein N is liberated to organize ammonium ions. Sulphuric acid oxides organic affair and it combines with the ammonium formed. The 2nd reaction consists of the sulfuric acid in the sample being neutralised with NaOH organizing ammonium hydroxide which is so distilled into a 4 % boracic acerb solution for the 3rd reaction. For the forth reaction a titration takes topographic point between the borate ions formed and the standardized 0.1M HCL until a pH of 4.6 is reached. Oz Moles of HCL= Moles of NH3 = Moles of N in sample. % Nitrogen x transition factor ( 6.38 ) = % protein Approximately 0.2g of Na caseinate pulverization was accurately weighed out and transferred into a Kjeldhal flask. Sucrose was used as a space. Into each flask 20mL of concentrated H2SO4 ( Low in N ) and two kjeldhal tablets. The samples were so placed in the Kjeldhal digestion unit ( Buchi, Labortechnik AO, Postfrach, Switzerland ) at 120Â °C for half an hr and temp increased to 420Â °C for 2 hours. During this clip digestion of organic affair in the sample occurs. After digestion the tubings are so cooled before being transferred to the Kjeldhal Buchi B323 Distillation unit for distillment and subsequent finding of protein content. This process was performed in triplicate. 2.3 Enzymatic hydrolysis of Na caseinate For the balance of this thesis the hydrolysate samples will be referred to in respects to protein concentration. Table 2.3.1 shows protein concentration in proportion to entire solids concentration for the Na caseinate hydrolysates generated at different protein/ entire solids concentrations. Table 2.3.1 Protein Concentration Entire Solids Concentration 5 % ( w/v ) 56.8g/L 10 % ( w/v ) 113.6g/L 15 % ( w/v ) 170.4g/L 20 % ( w/v ) 227.2g/L The enzymatic hydrolysis experiments were carried out in a 2L certain reaction vas ( ___ ) . The aqueous solutions of Na caseinate were allowed to hydrate for ~ 2hours at 50Â °C with the assistance of an overhead scaremonger. The solutions were so stored in a electric refrigerator at 4Â °C nightlong. On the twenty-four hours of the hydrolysis the solutions were equilibrated to 50Â °C and the pH was so adjusted to 7 by the add-on of 2N NaOH before the add-on of the enzyme. Prolyve 1000 was added at an enzyme: substrate ( Tocopherol: S ) ratio of 25ml enzyme readying / kilogram protein. The Tocopherol: S ratio was estimated on the footing of what has antecedently worked for whey, the standard add-on was 0.25mls of enzyme solution per 100ml to 10 % solution. The solution was assorted with an over caput scaremonger ( Heidolph Instruments, Schwabach, Germany ) and the pH was kept changeless throughout the hydrolysis utilizing a pH stat ( 718 Stat Titrino, Metrohm, Herisau, Switzerland ) . Hydrolysate samples were taken at assorted clip intervals, rapidly brought to 80Â °C utilizing a microwave and so maintained at 80Â °C for 20mins in a H2O bath to demobilize the enzyme. The samples were so stored at -20Â °C until required for analysis. 2.4 Quantification of Degree of Hydrolysis ( DH ) The TNBS reagent was made up of 0.1 % ( w/v ) TNBS in H2O. 1 % ( w/v ) SDS was used as the space and l-Leucine was used as the criterion. Samples and standard solutions were prepared in 1 % ( w/v ) SDS. The hydrolysate samples were diluted ( 1 in 51, 1 in 76, 1 in 101, 1 in 126, 1 in 151 A ; 1 in 201 in conformity with their protein concentrations ) . All samples were done in triplicate. 0.25 milliliter of the trial, integral Na caseinate ( control ) and l-Leucine criterion solutions was added to prove tubings incorporating 2.0mL of Na phosphate buffer ( 0.2125 m, pH 8.2 ) . The undermentioned stairss are light sensitive and took topographic point in the dark: 2 milliliter of TNBS reagent was so added to each tubing, followed by vortexing and incubation at 50AÂ °C for 60 min in a covered H2O bath. After incubation, the reaction was stopped by the add-on of 0.1N HCl ( 4.0 milliliter ) to each tubing. The samples were so allowed to chill in the dark at room temperature in order to st abilise the optical density readings. The optical density values were so read at 340nm utilizing a ( Carey 100 dual beam spectrophotometer ) The DH was calculated as follows: Where the N content of peptide bonds = 112.1 milligram of Nitrogen /g of protein for casein substrates. The protein concentration became marginally more dilute as NaOH was added throughout the hydrolysis reaction ; the protein concentration was calculated consequently. 2.5 RP-H.P.L.C. Reversed-phase HPLC was carried out on the Na caseinate hydrolysate samples utilizing a Waters HPLC system, consisting a Model 1525 binary pump, a Model 717 Plus autosampler and a Model 2487 double IÂ » optical density sensor interfaced with a BreezeTM data-handling bundle ( Waters, Milford, MA, USA ) . The column used was a Phenomenex Jupiter ( C18, 250r4.6 millimeter ID, 5 millimeter atom size, 300AAÂ ° pore size ) dividing column ( Phenomenex, Cheshire, UK ) with a Security GuardTM system incorporating a C18 ( ODS ) broad pore cartridge ( 4r3 millimeter ID, Phenomenex, Cheshire, UK ) . The column was equilibrated with dissolver A ( 0.1 % TFA ) at a flow rate of 1.0 ml min-1 and peptides were eluted with an increasing gradient of dissolver B ( 0. 1 % TFA, 80 % acetonitrile ) . TFA is used in HPLC as it improves symmetricalness of signals. Detector response was monitored at 214 nanometer A ; 280nm. The Na caseinate hydrolysate samples were diluted to 0.8 % ( w/v ) in 0.1 % TFA, filtered through 0.2 AÂ µm syringe filters and 20 AÂ µl was applied to the column. The undermentioned tabular arraies show the 3 different gradient profiles used in analyzing the samples. Basic contrary stage high public presentation liquid chromatography gradient profile Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 54.00 1.00 40.0 60.0 6 4 55.00 1.00 0.0 100.0 6 5 65.00 1.00 0.0 100.0 6 6 70.00 1.00 100.0 0.0 6 7 85.00 1.00 100.0 0.0 6 ( Table 2.5.1 ) Modified Profile 1 Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 84.00 1.00 40.0 60.0 6 4 85.00 1.00 0.0 100.0 6 5 95.00 1.00 0.0 100.0 6 6 100.00 1.00 100.0 0.0 6 7 115.00 1.00 100.0 0.0 6 ( Table 2.5.2 ) Modified Profile 2 Time Flow % A % B Curve 1 0.01 1.00 100.0 0.0 6 2 4.00 1.00 100.0 0.0 6 3 114.00 1.00 40.0 60.0 6 4 115.00 1.00 0.0 100.0 6 5 125.00 1.00 0.0 100.0 6 6 130.00 1.00 100.0 0.0 6 7 145.00 1.00 100.0 0.0 6 ( Table 2.5.3 ) The ultimate profile used on the samples with similar DH values but of different protein concentrations was the modified profile 2. 2.6 Centripetal In choosing campaigners for the gustatory sensation panel a acknowledgment trial for the four gustatory sensations was foremost performed. This involved doing up solutions of citric acid monohydrate ( rancid ) , sucrose ( sweet ) , caffeine ( acrimonious ) and NaCl ( salt ) all of which were made up in Ballygowan still H2O. At least two concentrations of each sample stand foring a gustatory sensation were included in the centripetal rating see table 2.6.1 Recognition Test Layout Sample Letter Concentration Chemical ( Taste ) A 0.02 % ( w/v ) Citric acid monohydrate ( Sour ) Bacillus 0.40 % ( w/v ) Sucrose ( Sweet ) C 0.03 % ( w/v ) Citric acid monohydrate ( Sour ) Calciferol 0.02 % ( w/v ) Caffeine ( Bitter ) Tocopherol 0.08 % ( w/v ) NaCl ( Salty ) F 0.60 % ( w/v ) Sucrose ( Sweet ) Gram 0.03 % ( w/v ) Caffeine ( Bitter ) Hydrogen Ballygowan still H2O Joule 0.15 % ( w/v ) NaCl ( Salty ) K 0.04 % ( w/v ) Citric acid monohydrate ( Sour ) ( Table 2.6.1 ) The campaigners were told that the samples contained natural Sweet, salty, rancid and acrimonious compounds. They were asked to savor each sample separately and bespeak whether the sample was sweet, salty, rancid or bitter in the provided infinites. If the sample tasted like H2O they were asked to tag with a nothing ( 0 ) , if they were unsure of the gustatory sensation they were asked to tag with a inquiry grade ( ? ) . When savoring the sample the campaigners were asked to twirl the solution around your oral cavity guaranting it contacts all parts of the lingua. Between samples, campaigners were asked to eat a piece of un-salted cracker and rinse their oral cavities exhaustively with still mineral H2O. A cut off point was set at 6 right replies, 1 of which must be acrimonious or 5 right replies, 2 of which must be acrimonious. Out of the 17 campaigners screened, 7 were selected for bitterness preparation. For the resentment preparation campaigners were asked to delegate bitterness tonss to unknown solutions based on a 0-100 % graduated table, where a 100 % acrimonious solution was taken to hold a resentment value equivalent to 1 g caffeine/L. Still mineral H2O was used as the 0 % bitterness criterion. All 7 displayed a strong ability to observe differen

Why Should You Use a Letter Essay Sample?

Why Should You Use a Letter Essay Sample?One of the most effective way to improve your academic performance is by writing a powerful essay. Whether you are attending college or are preparing to attend college, it is important that you are able to write an essay which will hold a high grade and that your essay reflects your personality. A letter essay sample can be very helpful when you want to be able to improve your academic skills.The letter essay sample that you will find on the internet is helpful for various reasons. First of all, it is an actual example of an essay that has been written. There are hundreds of people who are able to find essays on the internet which they can copy and modify, but the reason why many of them are not able to succeed is because they find it difficult to understand the meaning of the essay when they are only in the middle of writing the essay.When the student's paper has been reviewed by a professor, the professor will be able to provide suggestions that will be beneficial to the student's essay. This means that instead of only writing one example, as many students do, you should be able to compose many examples which will help to strengthen your intellectual abilities.Another good thing about a letter essay sample is that you can choose a word or two that you want to highlight in your essay. You may want to focus on the grammatical errors in the essay or you may want to focus on the punctuation mistakes. If you know that the editor or teacher will focus on, you will have fewer problems when you start writing your essay.One major problem that a lot of students face when they are writing essays is that they cannot get started because they are unsure of what they should write. It is also a problem that many students face when they are reading through their essays. If you are able to get a copy of a sample letter essay and then when you are writing your essay you can identify a few of the points that are highlighted, you will be i n a better position to figure out what you should say next.For example, if you look at the letter essay sample, you will see that there are several different paragraphs which are highlighted. If you can choose between a few of these paragraphs, you will be able to figure out which paragraph will be most effective.For the professor or the editor to review your essay, you need to be able to identify the parts of the essay that are most important. If you are able to identify these points, you will be in a better position to have a stronger essay.The letter essay sample is very helpful for students who are just starting their academic career. These types of samples to make it easier for the student to write a strong essay, but it also makes it easier for the student to identify and write a strong paragraph or even a strong paragraph. The best thing about these samples is that it is really a question of identifying what you need and then selecting the correct wording to say it.

The Bicycle Thief essays

The Bicycle Thief essays The Bicycle Thief by Vittorio De Sica is a movie that breaks the Hollywood aesthetic of beautiful people, fancy homes, and extensive wealth that only thrives in fantasy and sometimes-unconquerable dreams. It exploits the plight of working class individuals who live in what some may call ghettoes or over crowded cities. Standing outside of a government agency, men await the fates of their futures: will it be their day to get work or will they go home in defeat to tell their wives and children that they might not have enough money to eat? It is a travesty to envision a life that is lived moment to moment and the future holds no promise for a better tomorrow. It is striking to watch a movie that encapsulates the very heart of the working class. The audience is introduced to the movies central character, Antonio. A man who is in search of a job and the one he receives requires a mode of transportation common in those days, a bicycle. The bicycle he owns is in a repair shop waiting for alterations to be done, but because of the lack of money his family has, he never acquired the opportunity to pay for it. He then journeys back home to his wife, his son, and his newborn to herald the good news, however, as all good things too good to be true there is a catch. Antonio tells his wife that he can have this beneficial job if he has a bicycle, translation: we need money to get the bike so I can work. Maria, his wife, decides to pawn their precious linens, apart of her dowry, in order to retrieve Antonios bicycle from the shop. However, maintaining the bicycle long enough to see a single paycheck would pose as a dilemma rather than a means of support. First day on the job the bicycle gets stolen, crisis becomes the inevitable. Another plight of the working class individual, stolen goods are as good as gone and sold and money in some ...

Bachelor of Early Childhood Studies for Piaget

The four stage theory of Jean Piaget’s demonstrates the way through which children acquire knowledge through playing. This is one of the most critically criticized physiological theories of all decades. The theory on how children build up knowledge faced many challenges. Also many of them are pletely justified. Due to his grand vision the theory is fitting to round off this series on over 10 crucial child psychology studies along with the work of well known Swiss developmental psychologists Jean Piaget (Trevarthen et al., 2016).   His theory implies that, the intellect of a human develops with the help of assimilation, play and a modation. In the current field of technology the growing role of education system efficiently supports the understanding of the children. The nature of the technology encounters the need that helps to support the children to maximize their benefits. ICT in playground may be iPods, cell phones, MP3 players and puter are used to allow access to pictures, Video cameras and moreover games also. There are many ICT games available for the children such as Super Mario, puzzle, sketchpad, angry bird etc which helps them to explore their knowledge level. With the help of these ICT technologies the children can acquire high level knowledge. The stages mentioned in this theory include sensorimotor, pre operational, concrete operation and formal operation. While prising ICT and children play it has been found that,   puter games help them to b e more familiar with technology.   This theory reflects that children play active roles in the cultural and historical theory of human culture. In order to refine and test the basic practices of schooldays the children nature are analyzed by Lev Vygotsky (Hamari & Nousiainen, 2015). This theory implies that even without the help of the adults, the children can reach their desired destination only learning from the society. This means that after playing different games in the childhood, the children can learn new skills and ideas from the society. With the help of new learning skills and ideas without any assistance the children can reach highest peak of success. In current days children are also widely using technology in their daily lives. ICT development in the early childhood provides high quality practices that are helpful to support the children development and learning. In order to make these choices the practitioners need to be much familiar with different tools. With the application of puter games and online games the children can learn a lot from the social context. This is the theory of how the children learn self regulation through playing. The theory developed by Sigmund Freud, states that playing is the way that helps to express all the socially unacceptable behaviors (Moore, 2017). Play also helps a child to expel his or her undesired feelings and all the actions through a much acceptable direction. The sense of control of the children reduces anxiety and also helps to express all their forbidden feelings. Not only traditional games but advanced puter gaming helps children to obtain sense of control over different events. In the early childhood children can gain self regulation power through play. ICT plays effective role in the early childhood days. Early childhood education and play both include many hardware and software as their technology. Play can promote abstract ideas through creating separation between the objectives and actions. Using both the action and objects children can effectively reach even beyond the standard development in cognition and self regulation. Not only this but also from ICT context, children also obtain mental representations on social roles and about the rules of society for self regulation. The theory developed by Gregory Bateson implies the way through which the children learn munication through playing. It is focused on the script that the children follow while playing any game. munication reflects the ability of the child and also their ability to learn something new (Siegler, 2016). The munication approach shared among the children is termed as Meta munication skill. After considering the ICT aspect it can be said that, for any child munication is an important tool that has to be used to share thoughts and ideas. ICT technology offers deep social changes that influence segment of the human life and children. The characteristics of play are related to different practical activities. prising the ways that help the children to learn municate drives the evolution through enabling organisms for rapid adoption of novel environment. Gregory, E., Lytra, V., & Ilankuberan, A. (2015). Divine games and rituals: how Tamil Saiva/Hindu siblings learn faith practices through play.  International Journal of Play,  4(1), 69-83. Hamari, J., & Nousiainen, T. (2015, January). Why do teachers use game-based learning technologies? The role of individual and institutional ICT readiness. In  System Sciences (HICSS), 2015 48th Hawaii International Conference on  (pp. 682-691). IEEE. Moore, R. C. (2017).  Childhood's domain: Play and place in child development  (Vol. 6). Routledge. Roskos, K. A. (Ed.). (2017).  Play and literacy in early childhood: Research from multiple perspectives. Routledge. Siegler, R. S. (2016). Continuity and change in the field of cognitive development and in the perspectives of one cognitive developmentalist.  Child Development Perspectives,  10(2), 128-133. Trevarthen, C., Panksepp, J., Bentzen, M., Lakoseljac-Andreasen, C., Rubin, P. B., Winstead, M., ... & Goldstein, B. (2016).  Inclusion, Play and Empathy: Neuroaffective Development in Children's Groups. Jessica Kingsley Publishers.

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